Axonal Transport: Methods and Protocols

This volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo methods, written in the format of the highly successful Methods in Molecular Biology series. It aims to further our understanding of the trafficking of cellular components in the nervous system.

Format: Hardback
Length: 573 pages
Publication date: 13 April 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume spans a diverse array of model systems, encompassing invertebrates, humans, and both in vitro and in vivo contexts. It delves into detailed methods for studying various organisms, such as squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays. Written in the style of the renowned Methods in Molecular Biology series, each chapter begins with an introduction to the topic, outlines the necessary materials and reagents, provides helpful tips on troubleshooting and potential pitfalls, and presents step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Axonal Transport: Methods and Protocols serves as a vital resource in advancing our understanding of the intricate trafficking of cellular components within the nervous system. By presenting a wealth of information, this book aims to contribute to the advancement of fundamental knowledge that underpins the cell biology of neurons.

The chapters "Live Imaging of RNA Transport and Translation in Xenopus Retinal Axons," "Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons," "Assessment of Mitochondrial Trafficking as a Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons," "Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport," and "In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport" are available open access under a Creative Commons Attribution 4.0 International License via the link: link.springer.com.

These chapters offer valuable insights into the latest techniques and methodologies employed in studying axonal transport, providing researchers with essential tools to unravel the complexities of this vital process. Whether you are a novice or an experienced researcher, Axonal Transport: Methods and Protocols is an indispensable resource that will greatly assist you in your scientific endeavors.

Weight: 1295g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071619896
Edition number: 1st ed. 2022