DNA Damage Responses: Methods and Protocols

This volume provides detailed methods and key approaches used to study DNA damage, analyzing protein function, quantifying DNA replication dynamics, and biochemical reconstitution of key pathways involved in DNA repair. Written in the Methods in Molecular Biology series format, it aims to be a useful practical guide for researchers to help further their study in this field.

Format: Hardback
Length: 284 pages
Publication date: 16 March 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into intricate methods and essential approaches employed to mechanistically investigate DNA damage, encompassing the intricate factors involved in the damage response. Spanning multiple chapters, the book guides readers through diverse proteomics and biophysical techniques, enabling the analysis of protein function, quantification of DNA replication dynamics, and comprehensive evaluation of nucleic acid base damage. Additionally, it offers a detailed biochemical reconstitution of key pathways involved in DNA repair, providing a deep understanding of the intricate mechanisms at play. Written in the renowned Methods in Molecular Biology series format, each chapter begins with an introduction that provides a foundational understanding of the respective topic. It is followed by a comprehensive list of the necessary materials and reagents, accompanied by step-by-step, readily reproducible laboratory protocols. Furthermore, the book includes valuable tips on troubleshooting and avoiding common pitfalls, ensuring a smooth and successful experimental journey. Authored by esteemed experts in the field, DNA Damage Responses: Methods and Protocols strives to serve as an invaluable practical guide for researchers seeking to advance their knowledge in this dynamic and rapidly evolving field.

Weight: 765g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071620625
Edition number: 1st ed. 2022