DNA Manipulation and Analysis

This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification.

Format: Hardback
Length: 249 pages
Publication date: 01 March 2023
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into the intricate realms of recombinant DNA and nucleic acid manipulation, serving as a vital resource for researchers and scientists across the biological sciences. It provides detailed instructions and insights on how to successfully execute these technologies, empowering users to unlock the full potential of these powerful tools.

Chapters in this book guide the reader through the foundational aspects of oligonucleotide synthesis and DNA sequencing, paving the way for recombinant DNA plasmid work and cell-free experiments. Moreover, it offers up-to-date discussions on CRISPR approaches to genome modification, highlighting the latest developments in this rapidly evolving field.

Written in the renowned format of the Methods in Molecular Biology series, each chapter in Gene Modification and Nucleic Acid Technologies begins with an introduction to the topic, outlining the necessary materials and methods. Additionally, it includes helpful tips on troubleshooting and common pitfalls, ensuring a smooth and successful execution of the protocols. Furthermore, the step-by-step, readily reproducible protocols provided make it easy for readers to follow along and replicate the experiments in their own laboratories.

Authoritative and cutting-edge, Gene Modification and Nucleic Acid Technologies strives to be the go-to guide for life scientists venturing into the realm of recombinant DNA and nucleic acid manipulation. By offering a comprehensive and up-to-date exploration of these technologies, this book empowers researchers to push the boundaries of scientific discovery and advance our understanding of the complex mechanisms of life.

Weight: 693g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071630037
Edition number: 1st ed. 2023