DNA-Encoded Libraries

This book provides a comprehensive overview of DNA-Encoded Library (DEL) technology, which has emerged as an alternative to high throughput screening in drug discovery. It covers the entire DEL process, from conception to bench execution and clinical investigations, with contributions from experts in the field.

Format: Hardback
Length: 276 pages
Publication date: 26 November 2022
Publisher: Springer International Publishing AG

The book delves into the recent advancements in DNA-Encoded Library (DEL) technology, which has emerged as a promising alternative to high-throughput screening (HTS) in drug discovery. Over the past decade, DEL has gained significant attention and has been hailed as a disruptive technology due to its ability to efficiently screen large libraries of compounds and identify potential drug candidates.

The primary objective of this book is to provide a comprehensive and in-depth exploration of all the critical aspects of the DEL process, from its conceptualization to bench execution and clinical investigations. Renowned experts in the field have contributed their expertise, offering diverse perspectives from both academia and industry.

The book is designed to appeal to a wide range of readers, including researchers in the drug discovery field, graduate students, and scholars who are interested in this rapidly evolving technology. It offers a valuable resource for those seeking to gain a deeper understanding of DEL's capabilities, applications, and potential implications for drug development.

In the first chapter, the authors provide an introduction to DEL technology, highlighting its key features, advantages, and limitations. They discuss the historical background, theoretical foundations, and technological advancements that have contributed to the development of DEL. The chapter also provides an overview of the different types of DEL platforms available, including solid-phase, bead-based, and microfluidic systems.

Chapter 2 delves into the design and synthesis of DNA-encoded libraries. The authors discuss the principles of library design, including the selection of encoding elements, library size, and compound diversity. They explore the various encoding strategies, such as codon optimization, randomization, and combinatorial chemistry, that are used to enhance the screening efficiency and selectivity of DEL. The chapter also provides practical guidance on library preparation, including the synthesis of DNA constructs, library screening, and data analysis.

Chapter 3 focuses on the screening of DNA-encoded libraries using high-throughput screening platforms. The authors discuss the advantages and limitations of different screening methods, such as fluorescence-based screening, mass spectrometry-based screening, and cell-based screening. They explore the use of automated screening platforms, including robotic liquid handlers and high-throughput screening systems, to accelerate the screening process and improve the accuracy of hit identification. The chapter also provides practical tips and strategies for optimizing screening conditions and data analysis.

Chapter 4 explores the identification and characterization of hit compounds from DNA-encoded libraries. The authors discuss the methods used for compound identification, including high-resolution mass spectrometry, nuclear magnetic resonance spectroscopy, and bioassay-based screening. They explore the use of chemical informatics tools, such as structure-activity relationship (SAR) analysis and molecular modeling, to predict the biological activity and pharmacokinetic properties of hit compounds. The chapter also provides practical guidance of hit compounds, including lead optimization, structure-activity relationship (SAR) analysis, and preclinical studies.

Chapter 5 discusses the application of DNA-encoded libraries in drug discovery and development. The authors discuss the use of DEL in hit-to-lead optimization, drug discovery campaigns, and drug repositioning. They explore the use of DEL in personalized medicine, drug discovery for neglected diseases, and drug discovery for infectious diseases. The chapter also provides case studies and examples of successful applications of DEL in drug discovery.

Chapter 6 explores the challenges and limitations of DNA-encoded libraries in drug discovery. The authors discuss the challenges associated with library preparation, screening, and hit identification. They discuss the limitations of DEL in terms of compound diversity, scalability, and reproducibility. They also provide recommendations for addressing these challenges and improving the efficiency and effectiveness of DEL in drug discovery.

Chapter 7 discusses the future prospects and potential applications of DNA-encoded libraries in drug discovery. The authors discuss the ongoing research and development in DEL technology, including the development of new encoding strategies, library designs, and screening platforms. They explore the potential applications of DEL in other fields, such as materials science, biotechnology, and environmental science. The chapter also provides insights into the future trends and challenges in drug discovery and highlights the role of DNA-encoded libraries in meeting these challenges.

In conclusion, this book provides a comprehensive and up-to-date overview of DNA-Encoded Library (DEL) technology, its applications, and potential implications in drug discovery. The contributions from experts in the field offer diverse perspectives and insights, making it an invaluable resource for researchers, graduate students, and scholars interested in this rapidly improving technology. By exploring the critical components of the DEL process, from conception to bench execution and clinical investigations, this book aims to equip readers with the knowledge and skills necessary to leverage DEL's capabilities and contribute to the development of new drugs.

Weight: 600g
Dimension: 235 x 155 (mm)
ISBN-13: 9783031186288
Edition number: 1st ed. 2022