Epiblast Stem Cells: Methods and Protocols

This book provides up-to-date protocols for using epiblast stem cells to answer critical questions in mammalian development. It covers mouse primed pluripotent stem cells, characterization and study toolkits, and engineered gastrulation models. Written for the Method in Molecular Biology series, it offers step-by-step protocols, troubleshooting tips, and a guide to choosing the appropriate cell type for modeling the epiblast.

Format: Hardback
Length: 303 pages
Publication date: 30 April 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive book is meticulously designed to provide the most up-to-date protocols in utilizing epiblast stem cells (EpiSCs) to address crucial developmental questions in mammals. It begins by delving into the diverse spectrum of mouse primed pluripotent stem cells, paving the way for subsequent chapters that explore innovative toolkits for characterizing and studying these precious cells. The volume further extends its reach by delving into the realm of engineering gastrulation models using primed stem cells, offering valuable insights into the intricate processes of mammalian development. Authored by renowned experts in the field, each chapter in this book includes an informative introduction, a comprehensive list of necessary materials and reagents, meticulously detailed step-by-step laboratory protocols, and practical tips on troubleshooting and avoiding common pitfalls.

Moreover, Epiblast Stem Cells: Methods and Protocols stands as an invaluable resource for researchers seeking to overcome the reproducibility challenge by presenting a comprehensive collection of state-of-the-art protocols for conducting comprehensive analyses. This empowers readers to choose the appropriate cell type to model the epiblast, facilitating a deeper understanding of developmental processes and their underlying mechanisms.

In summary, this book is a must-have for scientists, researchers, and students interested in exploring the potential of epiblast stem cells in advancing our understanding of mammalian development. With its authoritative content, practical approach, and user-friendly format, it serves as an ideal guide for navigating the complexities of this field and achieving groundbreaking discoveries.

Weight: 794g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071622803
Edition number: 1st ed. 2022