Genome Editing in Animals: Methods and Protocols

The second edition of Genome Editing in Animals: Methods and Protocols provides new and updated protocols for generating knockout animals using three genome editing technologies. Written in the successful Methods in Molecular Biology series format, the chapters include introductions, lists of materials and reagents, step-by-step protocols, and troubleshooting tips.

Format: Hardback
Length: 399 pages
Publication date: 12 February 2023
Publisher: Springer-Verlag New York Inc.

This second edition provides new and updated protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Genome Editing in Animals: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.

Genome editing in animals has revolutionized the field of biology by allowing researchers to precisely modify the genetic makeup of animals. This second edition of Genome Editing in Animals: Methods and Protocols provides new and updated protocols that can be used for generating knockout animals.

Chapters in this book guide the reader through basic protocols for three genome editing technologies: CRISPR-Cas9, TALENs, and ZFNs. Each technology is discussed in detail, including its principles, applications, and potential limitations. The book also includes chapters on target design tools, which are essential for identifying the specific genes that need to be edited in order to achieve the desired outcome.

Specific protocols for each animal species are provided, covering a wide range of applications, such as gene knockout, gene insertion, and gene modification. These protocols are written in a step-by-step format, making them readily reproducible by researchers. Additionally, notes on troubleshooting and avoiding known pitfalls are included to help researchers overcome potential challenges during the genome editing process.

Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

This second edition of Genome Editing in Animals: Methods and Protocols is an authoritative and cutting-edge resource for researchers in the field of animal biology, genetics, and agriculture. It will be invaluable to researchers, students, and professionals who are interested in using genome editing technologies to advance their research and understanding of animal biology.

Weight: 969g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071630150
Edition number: 2nd ed. 2023