Hedgehog Signaling: Methods and Protocols

This book explores the technical breakthroughs with biophysical and cell biological approaches that have advanced the study of molecular mechanisms underlying the generation and transduction of the hedgehog (HH) signal, emphasizing the important role of sterols in this pathway. Written in the Methods in Molecular Biology series format, it provides researchers with a reliable reference for analyzing HH signal transduction through cell biological, biochemical, and biophysical approaches.

Format: Paperback / softback
Length: 244 pages
Publication date: 28 September 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive book delves into the groundbreaking advancements achieved through biophysical and cell biological approaches in the study of molecular mechanisms underlying the generation and transduction of the hedgehog (HH) signal. It highlights the significant role of sterols in this pathway. Within its pages, the volume explores various techniques involving key protein components, such as HH, the signaling ligand; Dispatched, a HH secretion regulator; Patched, the receptor of HH; Smoothened, the signal transducer responsible for transmitting the HH signal across the plasma membrane; and GLI, the transcription factor that activates HH target genes. Written in the popular Methods in Molecular Biology series format, chapters feature introductions to their respective topics, comprehensive lists of necessary materials and reagents, step-by-step readily reproducible laboratory protocols, as well as helpful tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Hedgehog Signaling: Methods and Protocols serves as a valuable reference for researchers in clinical and basic science seeking to analyze HH signal transduction through cell biological, biochemical, and biophysical approaches.

This comprehensive book delves into the groundbreaking advancements achieved through biophysical and cell biological approaches in the study of molecular mechanisms underlying the generation and transduction of the hedgehog (HH) signal. It highlights the significant role of sterols in this pathway. Within its pages, the volume explores various techniques involving key protein components, such as HH, the signaling ligand; Dispatched, a HH secretion regulator; Patched, the receptor of HH; Smoothened, the signal transducer responsible for transmitting the HH signal across the plasma membrane; and GLI, the transcription factor that activates HH target genes. Written in the popular Methods in Molecular Biology series format, chapters feature introductions to their respective topics, comprehensive lists of necessary materials and reagents, step-by-step readily reproducible laboratory protocols, as well as helpful tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Hedgehog Signaling: Methods and Protocols serves as a valuable reference for researchers in clinical and basic science seeking to analyze HH signal transduction through cell biological, biochemical, and biophysical approaches.

Weight: 493g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071617038
Edition number: 1st ed. 2022