Heterologous Expression of Membrane Proteins: Methods and Protocols

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies, including techniques using E. coli, mammalian cells, yeast, insect cells, and virus-like particles. It also includes new detergents and alternatives to detergents, and a chapter exploring the use of microscale thermophoresis to evaluate the binding activity of heterologously expressed proteins.

Format: Hardback
Length: 467 pages
Publication date: 01 July 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into protocols for the production of membrane proteins in a diverse panel of heterologous organisms, catering to structural studies. The book commences by exploring techniques employing Escherichia coli as a host for the overproduction and purification of membrane proteins. It proceeds with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as utilizing virus-like particles and acellular systems. Furthermore, the book introduces novel detergents and alternative detergent approaches that facilitate membrane protein purification for structural analyses. The volume concludes with a chapter examining the utilization of microscale thermophoresis (MST) to directly evaluate the binding activity of heterologously expressed proteins in crude membrane extracts. Authored for the esteemed Methods in Molecular Biology series, the chapters encompass introductions to their respective topics, comprehensive lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls.

Authoritative and timely, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an invaluable guide for scientists seeking to produce and purify functional recombinant membrane proteins for structural investigations.

Weight: 1107g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071623671
Edition number: 3rd ed. 2022