In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development

This handbook is a valuable resource for researchers and students in the field of anticancer drug discovery and development, providing them with a comprehensive and up-to-date guide to in vitro bioassay methods.
This handbook is a valuable resource for researchers and students in the field of anticancer drug discovery and development, providing them with a comprehensive and up-to-date guide to in vitro bioassay methods.

Format: Paperback / softback
Length: 116 pages
Publication date: 13 June 2022
Publisher: Taylor & Francis Ltd

This comprehensive and valuable handbook stands as a definitive and up-to-date compendium of essential in vitro bioassay methods employed to quantify and validate the anticancer activity of a drug candidate prior to its introduction into animal or clinical trials. In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development comprehensively explores the screening and evaluation of potential drug candidates across a diverse range of anticancer assays, highlighting the specific methodologies used by various pharmaceutical bioassays to interpret the activity of drug molecules. The primary focus of this book is to showcase bioassays that can be easily established and executed in any laboratory, regardless of limited funds, facilities, or technical expertise.

The first chapter provides an overview of the principles and applications of in vitro bioassays in anticancer drug discovery. It discusses the importance of these assays in identifying and characterizing potential anticancer agents, as well as their limitations and challenges. The chapter also highlights the various types of in vitro bioassays used, including cell-based assays, tissue culture assays, and molecular assays.

The second chapter focuses on the screening of potential anticancer agents using cell-based assays. It discusses the methods used to isolate and culture cells, as well as the techniques employed to evaluate the anticancer activity of test compounds. The chapter also covers the use of cell proliferation assays, cell cycle assays, and apoptosis assays to assess the efficacy of drug candidates.

The third chapter explores the evaluation of drug candidates using tissue culture assays. It discusses the methods used to establish and maintain cell cultures, as well as the techniques employed to evaluate the anticancer activity of test compounds. The chapter also covers the use of xenograft models, tumor necrosis factor receptor assays, and immunohistochemistry assays to assess the efficacy of drug candidates.

The fourth chapter focuses on the validation of anticancer drug candidates using molecular assays. It discusses the methods used to measure the expression of specific genes or proteins associated with cancer, as well as the techniques employed to evaluate the anticancer activity of test compounds. The chapter also covers the use of high-throughput screening methods, such as microarray analysis and next-generation sequencing, to identify novel anticancer drug targets.

The fifth chapter discusses the application of in vitro bioassays in drug development. It discusses the strategies used to optimize drug candidates for clinical trials, as well as the methods employed to evaluate the safety and efficacy of drug candidates in human subjects. The chapter also covers the use of preclinical models, such as animal models and cell-based models, to predict the clinical efficacy of drug candidates.

The final chapter provides an overview of the future trends in in vitro bioassay methods for anticancer drug discovery and development. It discusses the emerging technologies and techniques that are being developed to improve the efficiency and accuracy of these assays, as well as the challenges and opportunities that lie ahead.

In conclusion, In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development is a comprehensive and valuable resource for researchers, scientists, and pharmaceutical professionals involved in anticancer drug discovery and development. It provides a detailed and up-to-date overview of essential in vitro bioassay methods, highlighting their applications in screening, evaluating, and validating potential anticancer agents. The book is particularly useful for those working in laboratories with limited funds, facilities, or technical expertise, as it offers practical guidance on how to set up and execute bioassays in a laboratory setting. With its emphasis on accessibility and practicality, In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development is an indispensable tool for advancing the field of anticancer drug discovery and development.

Dimension: 234 x 156 (mm)
ISBN-13: 9781032339689