Lung Innate Immunity and Inflammation: Methods and Protocols

This book provides methods to isolate, characterize, and investigate key lung innate immune cells, with in vitro and in vivo model systems to study inflammatory lung diseases. It is written in the Methods in Molecular Biology series format and is intended to be a guidebook for researchers investigating innate immunity and inflammation in the lung and other organs and tissues.

\n Format: Hardback
\n Length: 433 pages
\n Publication date: 09 July 2018
\n Publisher: Humana Press Inc.
\n

This comprehensive book delves into intricate methods to isolate, characterize, and investigate vital lung innate immune cells. Providing a foundational overview, the volume proceeds to explore strategies for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the renowned Methods in Molecular Biology series format, chapters encompass introductions to their respective topics, comprehensive lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols serves as a valuable guidebook, appealing to researchers engaged in exploring innate immunity and inflammation in the lung, as well as other organs and tissues.

Isolation of Lung Innate Immune Cells:

The isolation of lung innate immune cells is a crucial step in understanding their role in lung inflammation and immunity. Various techniques have been developed to isolate these cells from lung tissue, including mechanical dissociation, enzymatic digestion, and cell sorting. Mechanical dissociation, such as using a cell scraper or homogenizer, is a simple and efficient method that can be used to isolate a large number of cells from lung tissue. However, it may also lead to cell damage and heterogeneity. Enzymatic digestion, on the other hand, allows for more precise isolation of specific cell types by targeting specific proteins or markers on the cell surface. This method is particularly useful for isolating immune cells, such as dendritic cells, macrophages, and neutrophils. Cell sorting, using flow cytometry or magnetic bead-based techniques, is a highly efficient method that allows for the isolation of specific cell populations based on their surface markers or intracellular proteins. This method is particularly useful for studying the heterogeneity of innate immune cells and their response to different stimuli.

Characterization of Lung Innate Immune Cells:

Once isolated, lung innate immune cells need to be characterized to understand their functional properties and their role in lung inflammation and immunity. Various methods have been developed to characterize these cells, including flow cytometry, immunohistochemistry, and molecular biology techniques. Flow cytometry is a powerful tool that allows for the simultaneous analysis of multiple cell markers and proteins on a single cell. It is particularly useful for studying the phenotypic and functional characteristics of immune cells, such as their surface markers, cytokine production, and cell-cycle progression. Immunohistochemistry is a technique that allows for the visualization of specific proteins or markers on tissue sections. It is particularly useful for studying the localization and expression of immune cells in lung tissue and for identifying the different cell types present. Molecular biology techniques, such as RT-PCR, Western blotting, and immunofluorescence, are used to study the expression of specific genes and proteins in immune cells. These techniques are particularly useful for studying the molecular mechanisms underlying the function of innate immune cells and for identifying potential biomarkers for lung inflammation and immunity.

Investigation of Lung Innate Immune Cells:

Once characterized, lung innate immune cells can be investigated to understand their role in lung inflammation and immunity. Various methods have been developed to investigate these cells, including in vitro assays, in vivo models, and clinical studies. In vitro assays are used to study the function of innate immune cells in a controlled environment. These assays can be used to study the response of immune cells to different stimuli, such as pathogens, allergens, and immune agonists. In vivo models are used to study the role of innate immune cells in lung inflammation and immunity in a more physiological environment. These models can be used to study the effects of immune cells on lung tissue and to identify potential targets for therapeutic intervention. Clinical studies are used to study the role of innate immune cells in lung inflammation and immunity in humans. These studies can be used to identify potential biomarkers for lung inflammation and immunity and to develop new therapeutic interventions for lung diseases.

Conclusion:

In conclusion, this comprehensive book explores methods to isolate, characterize, and investigate key lung innate immune cells. Providing a foundational overview, the volume proceeds to explore strategies for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the renowned Methods in Molecular Biology series format, chapters encompass introductions to their respective topics, comprehensive lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols serves as a valuable guidebook, appealing to researchers engaged in exploring innate immunity and inflammation in the lung, as well as other organs and tissues. By utilizing these methods, researchers can gain a deeper understanding of the role of lung innate immune cells in lung inflammation and immunity and develop new therapeutic interventions for lung diseases.

\n Weight: 972g\n
Dimension: 188 x 262 x 29 (mm)\n
ISBN-13: 9781493985692\n
Edition number: 1st ed. 2018\n