Mammalian Cell Engineering: Methods and Protocols

This book explores the latest engineering methods of mammalian cells to control their performance for future cell-based therapeutics and understanding complex biological systems. It is organized into five parts and includes CRISPR-Cas-based methods for genome editing, chemical compounds, and systems for controlling biological events.

Format: Paperback / softback
Length: 330 pages
Publication date: 07 July 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into the cutting-edge engineering techniques employed in mammalian cells, offering valuable insights into their potential applications in the field of cell-based therapeutics and the intricate study of complex biological systems. The book is organized into five distinct parts, each dedicated to exploring specific aspects of mammalian cell engineering.

Part One explores innovative methods to engineer mammalian cells to sense and respond to biologically relevant inputs, including cell-cell interactions and soluble proteins. By harnessing these sensory capabilities, researchers can develop cells that can detect and respond to environmental cues, paving the way for developing more sophisticated cell-based therapies and disease models.

Part Two delves into the realm of engineering mammalian cells to sense artificial inputs, such as light and ultrasound. These cells can be designed to respond to specific stimuli, enabling researchers to study biological processes in controlled environments and develop new imaging and sensing technologies.

Part Three presents cutting-edge CRISPR-Cas-based methods for highly multiplexed genome editing and spatiotemporally controlled genome editing. These techniques offer unprecedented precision and efficiency in modifying the genetic makeup of mammalian cells, opening up new avenues for studying gene function and developing personalized therapies.

Part Four explores the intersection of chemical compounds and systems with mammalian cell engineering. By combining chemical interventions with genetic engineering techniques, researchers can develop novel therapeutics and drug discovery platforms. This approach allows for the precise modulation of cellular processes and the development of targeted treatments for various diseases.

Part Five focuses on the engineering of specific mammalian cells in targeted manners. By manipulating cell differentiation, fate determination, and cellular signaling pathways, researchers can create cells with enhanced properties and functionalities for specific applications. This includes the development of stem cells, cancer cells, and cells for tissue engineering.

Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include comprehensive introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and helpful tips on troubleshooting and avoiding common pitfalls. Comprehensive and authoritative, Mammalian Cell Engineering: Methods and Protocols serves as a valuable resource for scientists seeking to advance their research in this field. By employing these cutting-edge engineering techniques, researchers can unlock the full potential of mammalian cells and contribute to the development of innovative therapeutics and biological systems.

Weight: 655g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071614433
Edition number: 1st ed. 2021