Membrane Trafficking: Methods and Protocols

This book provides a comprehensive collection of methods for studying membrane trafficking, including genetics, high-resolution imaging, in vitro biochemical and biophysical assays, and emerging topics beyond exocytic and endocytic pathways. Written for the Methods in Molecular Biology series, it is an ideal guide for junior researchers and established membrane biologists.

Format: Hardback
Length: 407 pages
Publication date: 13 July 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume offers a diverse range of methodologies for investigating membrane trafficking research, encompassing genetics, high-resolution imaging, in vitro biochemical, and biophysical assays. Spanning virtually all major trafficking branches, the book delves into the exocytic pathway, which involves cargo transport from the endoplasmic reticulum (ER) to the Golgi apparatus, through the Golgi cisternae, and to the plasma membrane and the extracellular space. Additionally, it explores the endocytic pathway, encompassing cargo endocytosis, endosomal recycling, and lysosomal degradation. Furthermore, the book addresses emerging topics beyond the conventional exocytic and endocytic pathways. Authored by renowned experts in the field, the chapters in this book include introductions to their respective topics, comprehensive lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls. Designed as part of the highly successful Methods in Molecular Biology series, this authoritative and practical resource serves as an ideal guide for both junior researchers embarking on their journey into membrane trafficking research and established membrane biologists seeking to expand their research programs.

Weight: 990g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071622087
Edition number: 1st ed. 2022