Natural Killer (NK) Cells: Methods and Protocols

This detailed book provides a broad collection of methodologies for natural killer (NK) cell research, including isolation, differentiation, functional tests, and clinical applications. Written for the Methods in Molecular Biology series, it is an ideal guide for researchers looking to contribute to the further development of NK cell research.

Format: Hardback
Length: 330 pages
Publication date: 29 March 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive book offers a diverse range of methodologies for studying natural killer (NK) cells. It begins by providing various techniques for isolating and differentiating NK cells, followed by comprehensive functional tests, including cytotoxicity, viral infection, and metabolism assays. Additionally, the volume explores the clinical applications of NK cells, highlighting their potential in immunotherapy and tumor detection. Written in the esteemed Methods in Molecular Biology series, each chapter includes an introduction, a list of necessary materials and reagents, step-by-step laboratory protocols, and helpful tips on troubleshooting and avoiding common pitfalls. Authored by esteemed experts in the field, Natural Killer (NK) Cells: Methods and Protocols serves as an invaluable resource for researchers seeking to advance their understanding of NK cell research, both at the basic and clinical levels.

Isolation and Differentiation of NK Cells:
The book begins by discussing the methods for isolating and differentiating NK cells from peripheral blood and other tissues. These techniques include cell sorting, flow cytometry, and immunomagnetic separation. The authors provide detailed protocols for each step, including the use of specific antibodies and reagents, as well as tips on optimizing the isolation and differentiation process.

Functional Tests:
The next section focuses on functional tests that can be performed on NK cells. These tests include cytotoxicity assays, which measure the ability of NK cells to kill target cells, and viral infection assays, which assess their antiviral response. Metabolism assays are also discussed, which help in understanding the metabolic pathways of NK cells and their role in immune regulation.

Clinical Applications:
The book then explores the clinical applications of NK cells. It discusses their role in immune-mediated diseases, such as autoimmune disorders and cancer, and their potential as immunotherapeutic agents. The authors provide examples of NK cell-based therapies in clinical trials and discuss the challenges and limitations of using NK cells in therapy.

Conclusion:
Natural Killer (NK) Cells: Methods and Protocols is a comprehensive and authoritative guide for researchers interested in studying NK cells. The book provides detailed protocols and insights into the isolation, differentiation, functional testing, and clinical applications of NK cells. Written by experts in the field, it serves as an invaluable resource for researchers seeking to contribute to the further development of NK cell research.

Weight: 845g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071621592
Edition number: 1st ed. 2022