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Non-Alcoholic Steatohepatitis: Methods and Protocols
Non-Alcoholic Steatohepatitis: Methods and Protocols
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- More about Non-Alcoholic Steatohepatitis: Methods and Protocols
This volume provides a comprehensive collection of laboratory protocols used to analyze non-alcoholic steatohepatitis (NASH). The chapters cover topics such as histological diagnosis, in vivo NASH model generation, hepatocyte and Kupffer cell isolation, human pluripotent stem cell-derived liver organoids, single-cell and RNA-sequencing, and exosomes and exosomal miRNAs extraction from mesenchymal stem cells. Written in the Methods in Molecular Biology series format, the book is a valuable resource for researchers studying NASH.
Format: Hardback
Length: 361 pages
Publication date: 26 February 2022
Publisher: Springer-Verlag New York Inc.
This comprehensive volume presents a diverse range of laboratory protocols employed by researchers to investigate various facets of non-alcoholic steatohepatitis (NASH). The chapters within this book delve into diverse topics, including methods for histological diagnosis of NASH, the rationale behind establishing an in vivo NASH model, protocols for isolating hepatocytes, Kupffer cells, bone marrow-derived macrophages, and adipocytes, techniques to generate human pluripotent stem cells-derived liver organoids, single-cell and RNA sequencing, and a detailed account of extracting exosomes and exosomal miRNAs from mesenchymal stem cells. Written in the renowned Methods in Molecular Biology series format, the chapters encompass introductions to their respective subjects, comprehensive lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Non-Alcoholic Steatohepatitis: Methods and Protocols serves as a valuable resource for both novice and experienced investigators engaged in studying NASH. Moreover, it serves as an indispensable reference for basic and clinical researchers, as well as students, in the field of NASH.
Weight: 910g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071621271
Edition number: 1st ed. 2022
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