Optical Tweezers: Methods and Protocols
Optical Tweezers: Methods and Protocols
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- More about Optical Tweezers: Methods and Protocols
This book provides detailed protocols for using optical tweezers, a technology that has become increasingly accessible to researchers, to study DNA-protein interactions, DNA motors, protein (un)folding experiments, actin- and microtubule-associated motor proteins, and the function and mechanical properties of microtubules and intermediate filaments. Written for the Methods in Molecular Biology series, it includes introductions, lists of materials and reagents, step-by-step protocols, and troubleshooting tips.
Format: Hardback
Length: 757 pages
Publication date: 06 September 2022
Publisher: Springer-Verlag New York Inc.
This comprehensive volume delves into a diverse range of techniques utilizing optical tweezers, a technology that has witnessed a remarkable increase in accessibility to a broad spectrum of researchers. The book commences by exploring recent technical advancements, paving the way for subsequent chapters that delve into the application of optical tweezers in studying DNA-protein interactions, DNA motors, protocols for conducting protein (un)folding experiments, the exploration of actin- and microtubule-associated motor proteins using optical tweezers, and protocols for investigating the function and mechanical properties of microtubules and intermediate filaments. Authored for the esteemed Methods in Molecular Biology series, the chapters encompass introductions to their respective topics, comprehensive lists of the necessary materials and reagents, detailed step-by-step laboratory protocols that are readily reproducible, and valuable tips on troubleshooting and avoiding known pitfalls.
Optical Tweezers: Methods and Protocols, Second Edition, stands as an exceptional resource for broadening the accessibility and utilization of optical traps by scientists from diverse disciplines. By presenting authoritative and practical insights, this edition serves as a vital tool for advancing the understanding and application of optical tweezers in scientific research.
Weight: 1630g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071622285
Edition number: 2nd ed. 2022
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