Peroxisomes: Methods and Protocols

A comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms is presented in this updated volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Format: Hardback
Length: 507 pages
Publication date: 24 March 2023
Publisher: Springer-Verlag New York Inc.

This fully updated volume presents a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in diverse organisms. Peroxisome research is experiencing a surge, with novel functions and proteins of this dynamic organelle continually being discovered through studies in model systems such as humans, mice, flies, plants, fungi, and yeast. This progress is evident in the chapters included in this collection, written for the renowned Methods in Molecular Biology series. Chapters encompass introductions to their respective topics, comprehensive lists of necessary materials and reagents, detailed step-by-step laboratory protocols that are readily reproducible, and practical tips on troubleshooting and avoiding known pitfalls. Authored by esteemed experts in the field, Peroxisomes: Methods and Protocols, Second Edition serves as an invaluable guide for researchers engaged in peroxisome- and organelle-based research inquiries.

Peroxisomes are small organelles present in eukaryotic cells, and their study has gained significant attention in recent years due to their important roles in various cellular processes. These organelles are involved in lipid metabolism, energy production, signaling, and the regulation of cellular homeostasis. In this comprehensive volume, we aim to provide a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms.

The first section of the book focuses on the isolation and purification of peroxisomes from various cell types. This includes methods such as density gradient centrifugation, flotation, and immunoprecipitation, which are essential for studying the structure, composition, and function of peroxisomes. The second section covers methods for studying the biogenesis and dynamics of peroxisomes. This includes techniques such as live cell imaging, fluorescence microscopy, and electron microscopy, which allow researchers to track the formation, movement, and fusion of peroxisomes in real time.

The third section explores the molecular biology of peroxisomes. This includes methods for studying the protein composition of peroxisomes, the regulation of peroxisome biogenesis, and the function of peroxisome-associated proteins. The fourth section focuses on the cellular functions of peroxisomes. This includes methods for studying the role of peroxisomes in lipid metabolism, energy production, signaling, and the regulation of cellular homeostasis.

The fifth section discusses the application of peroxisome research in various fields, such as medicine, agriculture, and biotechnology. This includes methods for studying the role of peroxisomes in diseases such as cancer, diabetes, and Alzheimer's disease, as well as methods for using peroxisomes in the development of new drugs and agricultural products.

In conclusion, this volume presents a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. By providing a detailed overview of the isolation, purification, biogenesis, dynamics, molecular biology, and cellular functions of peroxisomes, this book will be a valuable resource for researchers, students, and practitioners in the fields of biochemistry, cell biology, and medicine.

Weight: 1179g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071630471
Edition number: 2nd ed. 2023