piRNA: Methods and Protocols

This book provides detailed methods and protocols for studying piRNAs, including those developed for other ncRNAs involved in nuclear regulation, and those applied in non-classical organisms. It also includes bioinformatic and biophysical methods related to piRNA studies, reflecting the increasing importance of high-throughput sequencing and computational methods. Written for the Methods in Molecular Biology series, the chapters include introductions, lists of materials, step-by-step protocols, and tips on troubleshooting.

Format: Hardback
Length: 397 pages
Publication date: 08 July 2022
Publisher: Springer-Verlag New York Inc.

This extensive volume delves into the latest developments in the study of PIWI-interacting RNAs (piRNAs), methods employed for other non-coding RNAs involved in nuclear regulation that can be utilized to investigate piRNAs, and piRNA methodologies applied in non-classical organisms. It also encompasses a range of bioinformatic and biophysical approaches related to piRNA studies, aligning with the growing significance of high-throughput sequencing and computational methods. Comprising chapters authored by esteemed experts in the field, these chapters feature introductions to their respective topics, comprehensive lists of the necessary materials, detailed step-by-step protocols that are readily reproducible, and valuable tips on troubleshooting and avoiding common pitfalls. Serving as an invaluable resource for researchers seeking to unravel the numerous mysteries of this rapidly evolving area of multicellular biology, PIWI: Methods and Protocols stands as an authoritative and timely guide.

Weight: 969g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071623794
Edition number: 1st ed. 2022