Plant Proteases and Plant Cell Death: Methods and Protocols

This volume presents current methods to detect and measure the activity of proteolytic enzymes in organisms ranging from unicellular algae to flowering plants. It includes in vitro production, characterization of plant proteases, tools for in vivo modifications, proteomic approaches for identification of substrates, inhibitors, and interacting partners, and the function of plant proteases in plant programmed cell death. Written in the format of the highly successful Methods in Molecular Biology series, it aims to be a useful practical guide to researchers to help further their study in this field.

Format: Hardback
Length: 299 pages
Publication date: 19 May 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume explores innovative approaches to detect and measure the activity of proteolytic enzymes in a diverse range of organisms, spanning from unicellular algae to flowering plants. The chapters provide detailed insights into various methods employed for in vitro production, characterization of plant proteases, techniques for in vivo modifications, proteomic strategies for identifying substrates, inhibitors, and interacting partners, as well as the role of plant proteases in plant programmed cell death. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, a comprehensive list of necessary materials and reagents, practical tips on troubleshooting and potential pitfalls, and step-by-step, readily reproducible protocols. Authored by esteemed experts in the field, Plant Proteases: Methods and Protocols strives to serve as an invaluable practical guide for researchers seeking to advance their understanding in this dynamic and rapidly evolving area.

Weight: 787g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071620786
Edition number: 1st ed. 2022