Plant Secondary Metabolism Engineering: Methods and Protocols

This second edition provides practical information on methods used in engineering plant secondary metabolism pathways, including extraction, quantification, purification, localization, characterization, data mining, and biosynthesis modulation. Written in the format of the highly successful Methods in Molecular Biology series, it aims to be a useful guide for researchers working in this field.

Format: Hardback
Length: 262 pages
Publication date: 05 May 2022
Publisher: Springer-Verlag New York Inc.

This second edition offers comprehensive and practical guidance on essential methodologies employed in engineering plant secondary metabolism pathways. In addition to new and revised chapters, it provides readers with a thorough exploration of extraction, quantification, purification, localization, characterization, data mining, and processing techniques for various classes of plant specialized metabolites. Written in the style of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of necessary materials and reagents, helpful tips on troubleshooting and potential pitfalls, and step-by-step descriptions of readily reproducible protocols.

Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition serves as a valuable practical resource for researchers engaged in this dynamic field. By presenting detailed and up-to-date information, this edition aims to facilitate the advancement of research and development in plant biotechnology and medicinal chemistry.

The book is organized into nine chapters, covering a wide range of topics and techniques. Chapter 1 provides an overview of plant secondary metabolism, including its importance, classification, and potential applications. Chapter 2 focuses on extraction techniques, such as solid-phase extraction, liquid-liquid extraction, and supercritical fluid extraction, with a particular emphasis on the isolation of specialized metabolites from plant tissues.

Chapter 3 explores quantification methods, including high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and mass spectrometry (MS), for the analysis of specialized metabolites in plant samples. Chapter 4 discusses purification techniques, including column chromatography, size-exclusion chromatography, and preparative HPLC, with a focus on achieving high purity and yield of target compounds.

Chapter 5 explores localization techniques, such as immunohistochemistry, fluorescence in situ hybridization (FISH), and electron microscopy, for the identification and localization of specialized metabolites within plant cells and tissues. Chapter 6 delves into characterization methods, including nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and chromatography-based techniques, for the structural elucidation and identification of specialized metabolites.

Chapter 7 focuses on data mining and processing techniques, such as bioinformatics, statistical analysis, and machine learning, for the analysis and interpretation of large datasets generated from specialized metabolite research. Chapter 8 explores biosynthesis modulation techniques, including genetic engineering, metabolic engineering, and chemical synthesis, for the production of novel specialized metabolites with desired properties.

Chapter 9 discusses pathway engineering techniques, such as gene knockout, gene overexpression, and metabolic pathway reconstruction, for the optimization and manipulation of plant secondary metabolism pathways.

In conclusion, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition is a comprehensive and indispensable resource for researchers and practitioners seeking to advance their understanding and application of plant secondary metabolism. With its detailed coverage of methodologies, practical tips, and step-by-step protocols, this edition provides a valuable guide to facilitate the discovery and development of novel specialized metabolites with potential therapeutic and industrial applications.

Weight: 722g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071621844
Edition number: 2nd ed. 2022