Plasmodesmata: Methods and Protocols

This fully updated book aims to facilitate the study of plasmodesmata, nanochannels that connect plant cells, and to advance our knowledge of these structures. It covers general structural composition, regulation, and role in plant development and disease. The volume includes chapters on plasmodesmata architectures and distribution, composition, quantification, and research protocols. Written for the Methods in Molecular Biology series, it is an authoritative guide for plant scientists, particularly those studying cell-to-cell communication pathways.

Format: Paperback / softback
Length: 470 pages
Publication date: 13 April 2023
Publisher: Springer-Verlag New York Inc.

This extensively revised book serves as a valuable resource for the study of nanochannels, known as plasmodesmata, that connect plant cells. Its primary objective is to facilitate research and advance our understanding of these structures. The volume begins by providing a comprehensive overview of plasmodesmata's structural composition and regulation, including their role in plant development and disease. It then delves into chapters exploring plasmodesmata architectures and distribution in cell interfaces, methods for dissecting their composition, techniques to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, and a section dedicated to protocols that contribute to plasmodesmata research but fall outside previous classifications. Written for the renowned Methods in Molecular Biology series, each chapter includes an introduction to the respective topic, a list of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and helpful tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plasmodesmata: Methods and Protocols, Second Edition, serves as an essential guide for all plant scientists, regardless of their level of experience, particularly those interested in delving into the complexities of cell-to-cell communication pathways.

Weight: 913g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071621349
Edition number: 2nd ed. 2022