Practical Fluorescence Spectroscopy

This book provides a comprehensive and practical guide to fluorescence spectroscopy,covering basic concepts,experiments,and applications in biophysics,biochemistry,and the biomedical sciences. It includes nearly 150 experiments and emphasizes hands-on guidance and data interpretation.

Format: Paperback / softback
Length: 792 pages
Publication date: 14 June 2022
Publisher: Taylor & Francis Ltd

This comprehensive book offers a detailed and practical approach to fluorescence spectroscopy, covering both basic and advanced aspects of the field. It emphasizes hands-on experimentation, providing background information on fundamental concepts as well as guidance on handling artifacts, avoiding common errors, and interpreting data.

The book includes nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences, showcasing how fluorescence spectroscopy methods are applied in practical applications. These experiments cover a wide range of topics, from steady-state fluorescence to advanced time-resolved fluorescence, and demonstrate the versatility and importance of the technique in various scientific disciplines.

The book is organized into chapters that cover different aspects of fluorescence spectroscopy. The first chapter provides an introduction to the principles and techniques of fluorescence spectroscopy, including the basic principles of light absorption, emission, and fluorescence lifetime. The subsequent chapters discuss the theoretical background of fluorescence spectroscopy, including quantum mechanics, molecular dynamics, and chemical kinetics.

In the following chapters, the book presents laboratory methods using a variety of instrumental setups, such as fluorescence spectrometers, confocal microscopes, and flow cytometers. Each chapter includes detailed discussion of data analysis and interpretation, with examples and tips to help readers understand and apply the techniques effectively.

The book also covers steady-state phenomena, time-resolved phenomena, and advanced methods in fluorescence spectroscopy. It discusses the use of various excitation and emission wavelengths, the effects of environmental conditions on fluorescence signals, and the application of fluorescence spectroscopy to study biological structures, molecular interactions, and cellular processes.

In addition to the experimental chapters, the book includes a comprehensive section on commercially available instruments used for measurements in fluorescence spectroscopy. It provides information on the features and specifications of different instruments, as well as guidance on selecting the most appropriate instrument for a specific application.

Overall, this book is an invaluable resource for researchers and students interested in fluorescence spectroscopy. It provides a thorough and hands-on approach to the field, covering all the essential aspects from basic principles to advanced applications. With nearly 150 experiments and detailed discussion of data analysis and interpretation, it offers practical guidance and insights into the most important aspects of fluorescence spectroscopy.

Weight: 1470g
Dimension: 254 x 178 (mm)
ISBN-13: 9781032337371