Prokaryotic Gene Regulation: Methods and Protocols

This volume presents versatile methodologies to investigate prokaryotic gene regulation, covering the structure of the bacterial/archaeal chromosome, transcription initiation, regulating transcription factors, and the RNA-based level. It is written in the Methods in Molecular Biology series format and includes introductions, lists of materials and reagents, step-by-step protocols, and troubleshooting tips.

Format: Paperback / softback
Length: 318 pages
Publication date: 04 August 2023
Publisher: Springer-Verlag New York Inc.

Prokaryotic Gene Regulation: Methods and Protocols is a comprehensive volume that offers versatile methodologies for investigating prokaryotic gene regulation, with a particular focus on the different levels of information processing and their relevance for various model organisms, including archaeal and bacterial species. The book is divided into four sections, each addressing specific aspects of gene regulation. Section One delves into methods that enable the study of the bacterial/archaeal chromosome, its epigenetic modification, and the regulation of gene expression. Section Two explores various approaches to understanding transcription initiation, a crucial step in information processing. Section Three focuses on the investigation of transcription factors, which are essential regulators of gene expression in prokaryotic cells. Finally, Section Four explores the RNA-based level of gene regulation, which is crucial for the expression of genes. Written in the highly successful Methods in Molecular Biology series format, each chapter includes introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. This book is an invaluable resource for researchers interested in learning more about this diverse field.

Dimension: 254 x 178 (mm)
ISBN-13: 9781071624159
Edition number: 1st ed. 2022