Protein Arginylation: Methods and Protocols

This second edition provides updated and new chapters that detail comprehensive overview of all the existing methods on analyzing protein arginylation. Chapters guide readers through the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes.

Format: Hardback
Length: 297 pages
Publication date: 04 April 2023
Publisher: Springer-Verlag New York Inc.

This second edition offers updated and new chapters that provide a comprehensive overview of all existing methods for analyzing protein arginylation. The chapters guide readers through the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high-precision mass spectrometry, and chemical probes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Cutting-edge and thorough, Protein Arginylation: Methods and Protocols, Second Edition aims to be of interest to novice researchers involved in posttranslational arginylation research and researchers working on a broad range of posttranslational modifications.

Protein arginylation is a posttranslational modification that occurs on the amino acid arginine, where an arginine residue is modified by the addition of an arginyl group. This modification plays important roles in various biological processes, including cell signaling, protein stability, and protein function.

The analysis of protein arginylation is crucial for understanding the role of this modification in biological systems. Traditional methods for analyzing protein arginylation include Western blotting, immunohistochemistry, and mass spectrometry. However, these methods have limitations, such as low sensitivity and specificity, and require complex sample preparation.

In recent years, there has been a significant development in the methods for analyzing protein arginylation. These methods include recombinant protein techniques, antibodies, high-precision mass spectrometry, and chemical probes. Recombinant protein techniques involve expressing recombinant proteins in bacteria or yeast and then purifying the proteins to obtain pure samples for analysis. Antibodies are specific antibodies that recognize and bind to protein arginylation sites. High-precision mass spectrometry is a powerful tool for identifying and quantifying protein arginylation sites. Chemical probes are small molecules that can be used to label protein arginylation sites and detect their presence and location.

This second edition of Protein Arginylation: Methods and Protocols provides a comprehensive overview of all existing methods for analyzing protein arginylation. The chapters are organized in a logical and easy-to-follow manner, and each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

The book is written by experts in the field of protein arginylation research, and the chapters are authored by leading researchers from around the world. The book will be of great interest to novice researchers involved in posttranslational arginylation research and researchers working on a broad range of posttranslational modifications.

In conclusion, Protein Arginylation: Methods and Protocols, Second Edition is a valuable resource for researchers interested in analyzing protein arginylation. The book provides a comprehensive overview of all existing methods for analyzing protein arginylation, and the chapters are written by experts in the field. The book will be of great interest to novice researchers involved in posttranslational arginylation research and researchers working on a broad range of posttranslational modifications.

Weight: 787g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071629413
Edition number: 2nd ed. 2023