Proteoglycans: Methods and Protocols

This volume provides comprehensive and up-to-date strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs, in biological specimens, in vitro and in vivo effects, and compounds related with metabolic enzymes, epigenetic regulation, and PGs/GAGs-based inhibitors.

Format: Paperback / softback
Length: 317 pages
Publication date: 21 January 2024
Publisher: Springer-Verlag New York Inc.

Proteoglycans (PGs) and their glycan part, the GAGs, are essential components of the extracellular matrix (ECM) that play crucial roles in various biological processes. This volume provides a comprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of PGs and GAGs. The chapters are divided into three parts: GAGs in Biological Specimens, Protocols for the Evaluation of the In Vitro and In Vivo Effects of PGs/GAGs, and Protocols for Compounds Related with the Metabolic Enzymes, Epigenetic Regulation, and PGs/GAGs-Based Inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Cutting-edge and thorough, Proteoglycans: Methods and Protocols aims to provide information on the elucidated the structural and functional aspects of the complex matrix macromolecules such as the proteoglycans and glycosaminoglycans.

Dimension: 254 x 178 (mm)
ISBN-13: 9781071629482
Edition number: 1st ed. 2023