Recombineering: Methods and Protocols

This volume explores genome editing methods across bacteria, phages, and plants, including scarless DNA recombineering, HEMSE, Dup-In and DIRex, recombineering in Staphylococcus aureus, and genome editing with Cas9 in lactobacilli. Written in the Methods in Molecular Biology series format, it is a valuable resource for researchers interested in learning more about this developing field.

Format: Hardback
Length: 264 pages
Publication date: 18 May 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into a diverse range of methods employed in studying genome editing across various bacteria, phages, and plants. Within its chapters, readers will find in-depth discussions on topics such as scarless DNA recombineering in the lysogenic state of phages, HEMSE (homologous end-joining and single-stranded DNA repair), Dup-In and DIRex recombineering systems, recombineering in Staphylococcus aureus, and genome editing using CRISPR-Cas9 in lactobacilli. Written in the renowned Methods in Molecular Biology series format, each chapter begins with an introduction providing a foundational understanding of the respective topic. It then outlines the necessary materials and reagents required for experimentation, accompanied by step-by-step, readily reproducible laboratory protocols. Additionally, tips on troubleshooting and avoiding common pitfalls are provided to aid researchers in successfully executing the described procedures.

As a cutting-edge and thorough resource, Recombineering: Methods and Protocols serves as an invaluable tool for researchers seeking to expand their knowledge in this rapidly evolving field. Whether you are a novice or an experienced researcher, this book will provide you with the essential tools and techniques to explore and manipulate the genetic material of bacteria, phages, and plants.

Weight: 722g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071622322
Edition number: 1st ed. 2022