Revealing Uncharted Biology with Single Cell Multiplex Proteomic Technologies: Applications

Single-cell multiplex proteomic technologies have revolutionized biomedical research, enabling the analysis of protein abundance and activation states in individual cells. These platforms have led to the development of multiple technology platforms, profoundly impacting fields such as developmental biology, cancer biology, immunology, neuroscience, and drug discovery. This book focuses on the application of single-cell multiplex proteomic platforms to various biological systems, covering areas such as oncology, immunology, metabolomics, stem cell research, preclinical models, and translational research. It discusses incorporating these technologies into core facilities and consortia, providing access for multiple users and integrating datasets from other omics technologies. The following chapters cover applications in diverse areas such as muscle stem cell function in skeletal muscle regeneration, metabolic regulome profiling, translational studies, developing predictive biomarkers for patients receiving immune checkpoint inhibitors, and pre-clinical studies of lung cancer. These applications demonstrate how advanced single cell proteomic technologies are reshaping our understanding of complex biological systems and enhancing clinical translation.

Format: Paperback / softback
Length: 202 pages
Publication date: 27 June 2024
Publisher: Elsevier Science Publishing Co Inc

Single-cell multiplex proteomic technologies have revolutionized our understanding of biology, enabling us to explore the complex interplay between diverse cell types in healthy and diseased tissues. By analyzing the abundance and activation states of proteins in individual cells, we can identify key cell populations that contribute to disease pathogenesis and develop targeted therapies. Bulk protein analyses, on the other hand, mask critical differences between individual cells, making it difficult to understand the underlying mechanisms of disease. Moreover, the arrangement of cells into neighborhoods through cell-cell interactions is essential for tissue function, and single-cell proteomic phenotyping combined with positional information has become crucial for understanding biology in health and disease. This has led to the development of multiple technology platforms, profoundly impacting fields such as developmental biology, cancer biology, immunology, neuroscience, and drug discovery. This book focuses on the application of single-cell multiplex proteomic platforms to various biological systems, including oncology, immunology, metabolomics, stem cell research, preclinical models, and translational research. The initial chapters discuss incorporating these technologies into core facilities and consortia, providing access for multiple users and integrating datasets from other omics technologies. The following chapters cover application of single-cell multiplex proteomic technologies in various biological systems, including oncology, immunology, metabolomics, stem cell research, preclinical models, and translational research. The book highlights compelling studies where authors use these technologies to answer previously unanswerable questions and opens new avenues for scientific inquiry and clinical translation.

Weight: 440g
Dimension: 234 x 192 x 15 (mm)
ISBN-13: 9780128222133