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Ribosome Biogenesis: Methods and Protocols
Ribosome Biogenesis: Methods and Protocols
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Excellent- More about Ribosome Biogenesis: Methods and Protocols
This Open Access volume reviews the latest techniques to study eukaryotic ribosome biogenesis, which is essential for developing RNA-based vaccines like Covid-19. It covers various aspects, including genomic organization, in vitro methods, and translation analysis. Written in the Methods in Molecular Biology series format, the book is a valuable resource for scientists and researchers interested in learning more about in vitro RNA technologies.
Format: Paperback / softback
Length: 287 pages
Publication date: 08 July 2022
Publisher: Springer-Verlag New York Inc.
This Open Access volume presents comprehensive reviews and outlines the most recent techniques for studying eukaryotic ribosome biogenesis. For over 50 years, ribosomes have been a central focus of research. Our understanding of ribosome biogenesis and function, including transcription, mRNA modification, and translation, has been critical in developing powerful RNA-based vaccines against RNA viruses, such as the Covid-19 pandemic, which poses a global threat. The chapters in this book are organized into six parts. Part One explores a comparative survey of ribosome biogenesis in pro- and eukaryotic cells. Part Two delves into the genomic organization of eukaryotic rDNA and the role of RNA polymerase I in ribosomal RNA transcription. Part Three examines in vitro methods for studying RNA polymerase I structure and function. Part Four analyzes the nucleo-cytoplasmic transport of assembled ribosomes and RNP complexes. Part Five discusses modifications that increase the complexity of rRNAs. Part Six provides readers with a review of eukaryotic translation and, for the first time, describes a novel method for analyzing translation in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Ribosome Biogenesis: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about the growing importance of in vitro RNA technologies.
Weight: 581g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071625033
Edition number: 1st ed. 2022
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