SH2 Domains: Methods and Protocols

This book provides a comprehensive collection of techniques for studying SH2 domains and phosphotyrosine signaling, divided into six parts. It is a valuable resource for researchers interested in these areas, including computational biologists, biochemists, structural biologists, cell biologists, pathologists, and people interested in protein interaction domains and signaling systems.

\n Format: Paperback / softback
\n Length: 558 pages
\n Publication date: 09 June 2018
\n Publisher: Humana Press Inc.
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This comprehensive volume delves into a diverse range of techniques employed for studying SH2 domains and phosphotyrosine signaling. Organized into six distinct parts, the book provides a comprehensive overview of the field. Part I explores the historical background, technological advancements, and cell signaling mechanisms associated with SH2 domains. Part II delves into computational approaches and tools utilized for identifying, classifying, and predicting binding partners of SH2 domains. Part III details various methods for preparing SH2 domains as experimental reagents, enabling researchers to study their structural and functional properties. Part IV presents methods for conducting structural analysis and conventional binding assays using SH2 domains, providing valuable insights into their interactions and behavior. Part V highlights high-throughput and proteomics approaches to facilitate the analysis of SH2-mediated interactions on a large scale. Part VI explores the applications of SH2 domains in functional and imaging analyses, shedding light on their roles in various biological processes. Written in the renowned Methods in Molecular Biology series format, each chapter includes an introduction, a comprehensive list of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and helpful tips on troubleshooting and avoiding common pitfalls.

This book is a valuable resource for computational biologists, biochemists, structural biologists, cell biologists, pathologists, and individuals with an interest in SH2 domains and phosphotyrosine signaling. Researchers engaged in investigating the mechanisms by which protein interaction domains mediate specificity in signaling systems will also find this book informative.

\n Weight: 1050g\n
Dimension: 179 x 255 x 38 (mm)\n
ISBN-13: 9781493982950\n
Edition number: Softcover reprint of the original 1st ed. 2017\n