Somatic Cell Nuclear Transfer Technology

This volume provides protocols for animal cloning by Somatic cell nuclear transfer, aimed at basic research and biotechnological applications. Written in the format of the Methods in Molecular Biology series, it includes step-by-step, readily reproducible protocols and is a comprehensive guide for researchers.

Format: Hardback
Length: 320 pages
Publication date: 12 April 2023
Publisher: Springer-Verlag New York Inc.

This comprehensive volume provides detailed protocols for animal cloning through somatic cell nuclear transfer (SCNT) for both basic research and biotechnological applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, a comprehensive list of necessary materials and methods, helpful tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authored by esteemed experts in the field, Somatic Cell Nuclear Transfer Technology aims to serve as an authoritative and cutting-edge guide for researchers seeking to explore the potential of SCNT in their scientific endeavors.

The first chapter provides an overview of SCNT, including its historical background, theoretical principles, and potential applications. It discusses the various methods used for SCNT, such as electrofusion, microinjection, and cell-mediated fusion, and highlights the advantages and limitations of each approach. The chapter also provides an introduction to the tools and equipment required for SCNT, including cell culture media, transfection reagents, and imaging systems.

The second chapter focuses on the preparation of donor cells for SCNT. It discusses the methods used to induce pluripotency in somatic cells, such as retroviral-mediated expression of pluripotency factors, and describes the methods used to generate embryonic stem (ES) cells from pluripotent cells. The chapter also provides detailed protocols for the isolation and culture of animal cells, as well as the preparation of nuclear donor cells for SCNT.

The third chapter discusses the experimental procedures for SCNT. It outlines the methods used to combine the nuclear donor cell with the recipient egg, including the methods used to induce fusion and the timing of the fusion event. The chapter also provides detailed protocols for the culture of reconstructed embryos and the identification of transgenic animals.

The fourth chapter focuses on the applications of SCNT in basic research and biotechnological fields. It discusses the use of SCNT in the study of developmental biology, gene therapy, and tissue engineering. The chapter also provides examples of successful applications of SCNT in the production of transgenic animals, the development of pharmaceutical products, and the study of human diseases.

The fifth chapter discusses the ethical and legal considerations associated with SCNT. It discusses the potential risks and benefits of SCNT, as well as the ethical and legal issues surrounding the use of embryonic stem cells and the production of genetically modified animals. The chapter also provides guidelines for the responsible conduct of SCNT research and the dissemination of SCNT-related information.

The sixth chapter provides a comprehensive reference list of relevant literature and resources for researchers interested in SCNT. It includes a list of journals, books, and websites that provide information on SCNT, as well as a list of research centers and institutions that specialize in SCNT research.

In conclusion, Somatic Cell Nuclear Transfer Technology is a comprehensive and authoritative guide for researchers seeking to explore the potential of SCNT in basic research and biotechnological applications. Written in a clear and concise manner, the book provides detailed protocols for all stages of SCNT, from the preparation of donor cells to the culture of reconstructed embryos and the identification of transgenic animals. The book is an invaluable resource for researchers, students, and practitioners in the fields of biotechnology, genetics, and medicine.

Weight: 831g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071630631
Edition number: 1st ed. 2023