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Staphylococcus aureus: Methods and Protocols

Staphylococcus aureus: Methods and Protocols

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  • More about Staphylococcus aureus: Methods and Protocols

This book provides detailed phenotypic characterization of Staphylococcus aureus, focusing on in vitro and ex vivo methodologies. It covers topics such as virulence attributes, promoter activity, growth environments, metabolic and stress resistance assays, and host-pathogen interaction models. Written in the Methods in Molecular Biology series format, it is a valuable resource for researchers in the field.

Format: Paperback / softback
Length: 162 pages
Publication date: 17 July 2022
Publisher: Springer-Verlag New York Inc.


This comprehensive volume delves into the detailed phenotypic characterization of Staphylococcus aureus, emphasizing both in vitro and ex vivo methodologies. The chapters within this book encompass a wide range of topics, including in vitro evaluation of classical virulence attributes of S. aureus, quantification of promoter activity using a codon-optimized lacZ plasmid, establishment of biologically relevant growth environments, assessment of metabolic and stress resistance, and the use of in vivo and ex vivo models to study host-pathogen interactions. Written in the renowned Methods in Molecular Biology series format, each chapter begins with an introduction that provides an overview of the respective topic, outlines the necessary materials and reagents, presents step-by-step, readily reproducible laboratory protocols, and offers valuable tips on troubleshooting and avoiding common pitfalls. With its cutting-edge and authoritative content, Staphylococcus aureus: Methods and Protocols serves as an invaluable resource for researchers and practitioners interested in exploring the intricate aspects of this fascinating and rapidly evolving field.

Weight: 339g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071615522
Edition number: 1st ed. 2021

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