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The P2X7 Receptor: Methods and Protocols
The P2X7 Receptor: Methods and Protocols
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- More about The P2X7 Receptor: Methods and Protocols
The P2X7 Receptor: Methods and Protocols is a comprehensive volume that covers diverse aspects of P2X7 receptor analysis, including its molecular structure, ligands, physiological functions, generation of antibody and nanobody tools, electrophysiological analysis, and investigation of P2X7 down-stream signaling in immune cells. Written for the highly successful Methods in Molecular Biology series, the book provides step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Format: Hardback
Length: 394 pages
Publication date: 02 July 2022
Publisher: Springer-Verlag New York Inc.
This extensive volume delves into diverse aspects of P2X7 receptor analysis, encompassing its molecular structure, pharmacological and immunological tools, heterologous expression systems, and assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Additionally, mouse models and procedures tailored to investigate P2X7-mediated effects in other primary cells and in vivo are explained. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The P2X7 Receptor: Methods and Protocols is a valuable reference not only for the growing community fascinated by this unusual ion channel but also for a broad readership interested in ion channels or purinergic receptors.
Weight: 961g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071623831
Edition number: 1st ed. 2022
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