Translational Research Methods in Neurodevelopmental Disorders

This volume explores the latest techniques used to study neurodevelopmental diseases (NDD) that range from molecular aspects to integrated research approaches and brain imaging in living rodents. Chapters include protocols to deliver shRNA in vitro and in vivo, recombinant fluorescent probes, CRISPR/Cas9 toolkit, in vivo manipulations, and genetic engineering macaque models. It is a valuable resource for researchers who want to learn more about this important and developing field.

Format: Hardback
Length: 264 pages
Publication date: 30 September 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into the latest techniques employed to study neurodevelopmental diseases (NDDs), encompassing a wide range of approaches from molecular aspects to integrated research methodologies and brain imaging in living rodents. The book's chapters cover a diverse array of topics, including protocols for delivering shRNA in vitro and in vivo using lentiviral particles to knock down specific protein expression, experimental procedures for visualizing endogenous proteins at the mammalian synapse using recombinant fluorescent probes, the CRISPR/Cas9 toolkit for assessing gain- or loss-of-gene function in brain organoids, detailed protocols for in vivo manipulations to correct behavioral phenotypes in cognitive disorder mouse lines, and experimental approaches to genetically engineer macaque models of NDDs to investigate the potential effects of genetic predisposition on neural and functional alterations. In the hallmark style of the Neuromethods series, chapters provide in-depth detail and valuable guidance from experts, ensuring that researchers can achieve successful outcomes in their laboratory endeavors.

Translational Research Methods in Neurodevelopmental Disorders serves as a valuable resource for all researchers seeking to expand their knowledge in this crucial and rapidly evolving field. By presenting cutting-edge and practical techniques, this book empowers researchers to explore the complexities of NDDs and contribute to the development of innovative therapies and interventions.

Weight: 729g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071625682
Edition number: 1st ed. 2022