Yeast Surface Display

Yeast Surface Display is a comprehensive resource that provides detailed protocols for constructing and screening yeast surface display libraries, selecting libraries for binding to mammalian cells or extracellular matrix, and performing specialized applications. Written for the Methods in Molecular Biology series, the book is authoritative and practical and enables the implementation of this powerful technique in any molecular biology laboratory.

Format: Hardback
Length: 646 pages
Publication date: 29 April 2022
Publisher: Springer-Verlag New York Inc.

This extensive volume delves into a diverse range of applications of yeast surface display, a widely employed protein engineering technology. The book begins by providing comprehensive protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants. It proceeds with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix, showcasing the versatility of this display platform. Written for the esteemed Methods in Molecular Biology series, the chapters encompass introductions to their respective topics, comprehensive lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and valuable tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as an indispensable resource that empowers researchers to implement this powerful and versatile technique in virtually any molecular biology laboratory, regardless of prior experience with yeast surface display.

Weight: 1429g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071622841
Edition number: 1st ed. 2022